Busch Lab

ZMP

polr2a

Ensembl ID:
ENSDARG00000076177
ZFIN ID:
ZDB-GENE-041008-78
Description:
Polr2a protein [Source:UniProtKB/TrEMBL;Acc:Q58EF0]
Human Orthologue:
POLR2A
Human Description:
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa [Source:HGNC Symbol;Acc:9187]
Mouse Orthologue:
Polr2a
Mouse Description:
polymerase (RNA) II (DNA directed) polypeptide A Gene [Source:MGI Symbol;Acc:MGI:98086]

Alleles

There are 7 alleles of this gene:

Allele Name Consequence Status Availability
sa35290 Nonsense Mutation detected in F1 DNA Not yet available
sa22095 Nonsense Available for shipment Available now
sa18581 Nonsense Available for shipment Available now
sa38889 Nonsense Mutation detected in F1 DNA Not yet available
sa35291 Nonsense Mutation detected in F1 DNA Not yet available
sa42025 Nonsense Mutation detected in F1 DNA Not yet available
sa42026 Nonsense Mutation detected in F1 DNA Not yet available

Mutation Details

Allele Name:
sa35290
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
C > T
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000077495 Nonsense 289 1965 6 28
Genomic Location (Zv9):
Chromosome 12 (position 23918277)
Other Location(s):
Assembly Chromosome Position
GRCz10 12 22441595
GRCz11 12 22562814
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
ATTTAACACACAAGTTGGCTGACATTGTCAAGATCAATAACCAGCTGAAG[C/T]GAAATGAGCAGAGTGGAGCAGCAGCTCATGTTATAGCAGAGGATGTCAAG
Long Flanking Sequence:
TAGCCTTTGTTGCTGACATAACATTCAAAAATACATTACTTTTACTGTACGTCTTCAGGGTCATGGAGGCTGTGGCAGGTACCAACCACGCATCCGTCGCTCTGGCCTGGAGTTGTATGCTGAATGGAAGCATGTGAATGAAGACTCTCAGGAGAAAAAGATTCTCCTCAGCCCTGAACGTGTGCATGAGATCTTCAAGCGCATTGCAGATGAAGAGGACATAATCCTGGGCATGGACCCTAAATACGCCCGTCCCGAGTGGATGATCGTCACAGTGTTGCCTGTTCCCCCTCTAGCTGTCAGACCGGCTGTGGTCATGCAGGGCTCTGCTAGGAACCAGGTTAGTATTTCTTTGTAGCCAGAGGGAAAGTGGGCAGGTGTACCGCAAGGGTTAGTACTGATGACTGTTAGGTTTGATTCAATTGTATGTTTTCACCTTCCACCAGGATGATTTAACACACAAGTTGGCTGACATTGTCAAGATCAATAACCAGCTGAAG[C/T]GAAATGAGCAGAGTGGAGCAGCAGCTCATGTTATAGCAGAGGATGTCAAGCTGCTTCAGTTTCACGTGGCCACCATGGTGGACAATGAACTGCCAGGTCTACCTAGGGTAAGATTTACAGTATAACTCAGATGATGTTTATACACTTTGTAATGGTTATATGCTTATTAACTGGCATGATTTGTTAATGTAGTAGTGTTTATTAATGTTTTGTTAGTGATTGTAAATATAATTTATTGTAAATATATTTATTGTAAATTTTAGGTAGTTGTAATTGTGTAAAAGTAAAAAATGCAGCCTTGTCTATATAAAAATAGATGTTAGAATGCAATGTTTTAGTTTTTTACTTTAAGTGAATCAAACGTTTTTTGTTTTTTTTTTACCATGATTTAAAGAAGAGACACACACTGTCTGGGTGGCCTTTTATAGAATATTAATTTGACAGATTTAAAGGCTTTTCTATTTGGGTTTCTATTCGCAGTGCAGCAGTAATATACTAGT
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa22095
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Order Allele From ZIRC
Mutation:
C > T
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000077495 Nonsense 708 1965 13 28
Genomic Location (Zv9):
Chromosome 12 (position 23921025)
Other Location(s):
Assembly Chromosome Position
GRCz10 12 22444350
GRCz11 12 22565569
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
CTGATAAGGCGACATATCAGGACATTCAGAACACTATCAAGAAAGCCAAA[C/T]AGGATGTGATAGAGGTGCGGTGGCATTGTTACTAAATATCAAGGCTCTGT
Long Flanking Sequence:
GAGGACAGTGGCCCTTACAAGAACATCTCCCCTGGAGACACAAAGGTGCAGTAGTGTAGCCCAAGCATGGGCATATTTATGCCGTTATTTTTTATTTTTATATATATATTTTTTTTTGATTATTCACTTAATTTTTTGTGTATTTTTGCAGGTAATTGTGGAGAACGGTGAGCTGATCATGGGGATCCTGTGTAAGAAATCGTTGGGAACCTCAGCTGGATCACTTGTCCATATCTCATACCTTGAGATGGGCCATGACATCACAAGACTCTTCTATTCCAACATCCAGACTGTGGTTAACAACTGGCTCCTCATTGAAGGTAAAATAAGCAAATTGAGTTTGAAGGTTTTTCTCCTAATCAGGCTGATATTAAGTTCTAATCAATACGGTGTTTGTTCTTTTCTTCTTGTCTGCTTTAGGTCACTCTATTGGTATTGGAGACTCCATTGCTGATAAGGCGACATATCAGGACATTCAGAACACTATCAAGAAAGCCAAA[C/T]AGGATGTGATAGAGGTGCGGTGGCATTGTTACTAAATATCAAGGCTCTGTTATAAGACGCTTGTGCACATGTTGACTTATTTTTCTGCTTCTGTTCTGCTCACAGGTCATTGAAAAAGCCCACAACAATGAGCTGGAGCCTACTCCAGGTAACACTTTGAGACAGACCTTTGAGAACCAGGTTAATCGCATTCTGAATGATGCTCGAGACAAAACTGGATCCTCTGCCCAGAAGTCCCTGTCAGAGTACAACAATTTCAAATCCATGGTGGTGGCTGGTTCAAAAGGGTCCAAAATTAACATTTCTCAGGTGAGAGCAGTCTTCCTTGTTTTAATGTTTAAAATTTTAATGGGGCGTTCATTGCCGACAACTCAATTTTAACCCTAAACCAGAAATGAGACTTTCTAAAAATGTACATGCAAATATTGTCAGATATCATAAATTTTGGTCTTTTATGCTTTTTCTGTTCACCCGTTTAGGTTATTGCTGTGGTGGGTCAG
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa18581
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Order Allele From ZIRC
Order Allele From EZRC
Mutation:
C > T
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000077495 Nonsense 925 1965 17 28
Genomic Location (Zv9):
Chromosome 12 (position 23922284)
Other Location(s):
Assembly Chromosome Position
GRCz10 12 22445605
GRCz11 12 22566824
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
TCCACCTCTTTTTCCAATCACACAGGTTCAAGTTTGACTACGCCAATGAA[C/T]GAGCTCTCCGCCGCACTCTTCAGGAAGATGTGGTGAAAGATGTGATGACC
Long Flanking Sequence:
TATATTATATGAAACTGTCAAAATGTCATCAGGTTTGTCTCCATGATTTTAATTTTTCTACTGAATGTCAGGTTATATTCAGCGTCGTCTGATCAAGTCTATGGAGTCTGTTATGGTGAAGTACGATGCAACAGTCAGAAACTCCATTAACCAGGTTGTCCAGCTGAGGTATGGAGAGGATGGGCTTGCAGGAGAGGCTGTGGAGTTTCAAAATATGGCCACTCTTAAGCCATCGCACAAAGCCTTTGAAAAGAAGTGAGTTTTTTGCATACATTTTGACAGTTAAGTATAAATGTCCAGTATTTTAAATTATATATAAAATGGCTAAATTGCCCTAAATCAAAAGGTTTAAAATTGTTATCACTTGTATCGTTTAAGTCATTTTAGGTCTTTGTTGATAGCCGATAGCGAGTGCCCACGTGATGTATACAAATGTCACTGATTCAACTGTCCACCTCTTTTTCCAATCACACAGGTTCAAGTTTGACTACGCCAATGAA[C/T]GAGCTCTCCGCCGCACTCTTCAGGAAGATGTGGTGAAAGATGTGATGACCAATGCTCATGTCCAGAGTTCTCTAGAGAGAGAGTTTGAGAAGATGAGGGAAGACCGAGAGATCTTGAGGGCCATTTTCCCCACAGGAGACAGCAAGGTCTGTAACATTAAGCACCATCAATGCCCAAACACATGAACATCATTGGATATTTTGTAAATGTCTGAGGTGTGCAAATATAAAGACACAAACTGCTATGTTTTCTATAGGTGGTCCTACCGTGCAATCTGGCCAGAATGATCTGGAATGCTCAGAAGATTTTCCGCATTAACCCTCGGGCACCAACTGATCTCAACCCAGCACGGGTTGTGGACGGTGGGTTCAAAACATTGGTTTGTGGTTTTAAAAATCTGTAAATGTTAGATTTATTGTTTGATATCTTATGATATCTTTATTTTGAAATATCTTTATTTTAACATGCACTTTAAGAGACTGTGTCTGACTGGGTCTCCC
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa38889
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
T > A
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000077495 Nonsense 1013 1965 19 28
Genomic Location (Zv9):
Chromosome 12 (position 23923002)
Other Location(s):
Assembly Chromosome Position
GRCz10 12 22446311
GRCz11 12 22567530
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
ATAGTTCATTAAATTTTCCTCTGCTTTTATTTTTGTAGGAGTTAAGGAGT[T/A]GAGTAAGAAGCTGGTGATTGTAAATGGCGATGACCAGTTAAGCAGGCAAG
Long Flanking Sequence:
GTGCAAATATAAAGACACAAACTGCTATGTTTTCTATAGGTGGTCCTACCGTGCAATCTGGCCAGAATGATCTGGAATGCTCAGAAGATTTTCCGCATTAACCCTCGGGCACCAACTGATCTCAACCCAGCACGGGTTGTGGACGGTGGGTTCAAAACATTGGTTTGTGGTTTTAAAAATCTGTAAATGTTAGATTTATTGTTTGATATCTTATGATATCTTTATTTTGAAATATCTTTATTTTAACATGCACTTTAAGAGACTGTGTCTGACTGGGTCTCCCAGCTAAATAAAGACTTTGTGCCATCTATATTAAGCCTGCTGAAGACTGATCCCTATAGCTTACTGCTGGCTTGTGGCAGACCTGTGCAAGCTGATTATAAAGTCTCAGCTTTTGCATCATTCTATACATTGACTAAAGCAAAACAAAACATTTGCTCTATTGTTATGATAGTTCATTAAATTTTCCTCTGCTTTTATTTTTGTAGGAGTTAAGGAGT[T/A]GAGTAAGAAGCTGGTGATTGTAAATGGCGATGACCAGTTAAGCAGGCAAGCCCAGGAGAACGCCACTCTGCTTTTCAACATCCATCTGCGATCAACCCTCTGTTCCAAACGAATGACTGAGGAGTTCCGTCTTAGCACTGAGGCCTTTGATTGGCTGCTGGGAGAGATTGAGACCAAATTTAACCAATCCATTGTAAGTGAATGCATTCTGAGTGCAGCTGAATGTCTGCACCTGGATAAATTGGAAATATTGAGCAAAATCAAATTGATACAATTTTAAAAGTGCCTTCATTGACTGACGCTGTACATTTTTCTTTTCCGTTTACCTCAGGCTCACCCAGGTGAGATGGTAGGTGCTCTGGCTGCCCAGTCCCTGGGAGAGCCTGCTACTCAGATGACCCTGAACACATTCCACTACGCCGGTGTGTCCGCCAAAAACGTCACTCTCGGTGTGCCTCGTCTCAAAGAGCTTATCAACATCTCCAAGCGACCCAAGACCC
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa35291
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
T > G
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000077495 Nonsense 1174 1965 20 28
Genomic Location (Zv9):
Chromosome 12 (position 23923624)
Other Location(s):
Assembly Chromosome Position
GRCz10 12 22446933
GRCz11 12 22568152
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
TTGGAGCACACAACCCTGCGCAAGGTCACTGCCAACACAGCCATTTATTA[T/G]GACCCCAACCCTCAGAACACTGTGGTGACTGAGGATCAAGAGTGGGTTAA
Long Flanking Sequence:
GAGTTCCGTCTTAGCACTGAGGCCTTTGATTGGCTGCTGGGAGAGATTGAGACCAAATTTAACCAATCCATTGTAAGTGAATGCATTCTGAGTGCAGCTGAATGTCTGCACCTGGATAAATTGGAAATATTGAGCAAAATCAAATTGATACAATTTTAAAAGTGCCTTCATTGACTGACGCTGTACATTTTTCTTTTCCGTTTACCTCAGGCTCACCCAGGTGAGATGGTAGGTGCTCTGGCTGCCCAGTCCCTGGGAGAGCCTGCTACTCAGATGACCCTGAACACATTCCACTACGCCGGTGTGTCCGCCAAAAACGTCACTCTCGGTGTGCCTCGTCTCAAAGAGCTTATCAACATCTCCAAGCGACCCAAGACCCCCTCCCTGACTGTCTTTCTACTGGGCCAAGCAGCCCGTGATGCAGAGAGGGCCAAAGATATCCTGTGTCGGTTGGAGCACACAACCCTGCGCAAGGTCACTGCCAACACAGCCATTTATTA[T/G]GACCCCAACCCTCAGAACACTGTGGTGACTGAGGATCAAGAGTGGGTTAACGTCTACTATGAGATGCCTGACTTCGATGTGACCCGAATTTCACCCTGGCTGTTGCGTATCGAACTTGACCGCAAACACATGACTGACCGTAAGCTGACTATGGAGCAAATTGCAGAAAAGATCAATGCAGGTGGGTCTGATATTAGTGAATAGACATACTTCTAACACTTTTAACGGGCTATCAAATTTTAATGCTTGTCTGTGTGTTAGGATTTGGTGACGACTTAAACTGCATCTTCAATGATGACAATGCTGAGAAACTGGTACTGCGAATCCGCATCATGAACAGCGACGAGAACAAGTTCCAAGAGGTGTTGTAATTTAACATTTTTATTGGTGCATGAGCATAACTGTCTACTTTCTCTCAATGTTGAATTCGAAATCTTGTCACCAGGATGAGGAGGTTGTAGATAAGATGGACGATGATGTCTTCCTTCGATGCATTGAAT
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa42025
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
G > A
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000077495 Nonsense 1331 1965 23 28
Genomic Location (Zv9):
Chromosome 12 (position 23924454)
Other Location(s):
Assembly Chromosome Position
GRCz10 12 22447763
GRCz11 12 22568982
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
AAGAAAATCATCATCACAGAAGATGGAGAGTTTAAAGCCCTGCAGGAGTG[G/A]ATCCTGGAAACAGATGGAGTCAGTCTCATGAGGGTCCTTAGTGAAAAGGA
Long Flanking Sequence:
ATCATGAACAGCGACGAGAACAAGTTCCAAGAGGTGTTGTAATTTAACATTTTTATTGGTGCATGAGCATAACTGTCTACTTTCTCTCAATGTTGAATTCGAAATCTTGTCACCAGGATGAGGAGGTTGTAGATAAGATGGACGATGATGTCTTCCTTCGATGCATTGAATCCAACATGCTGACAGACATGACCCTGCAAGGCATTGAGCAGATCAGCAAGGTATACCTCTTTTGGCAGTTTGGCTTCTATGCTGTCTAATACCCGACAGTCCGTTTGGCAGTGCAGGCTTGTCATAAATGACACTTGTATGCACTTCAGACAGCTAATTGGTACCCACCTGGATTATAAAGTGACAATTGGAAGGGCCTACTGTTTTGTGGATGAAATTTAAGTTTCTCCCTCTCTACACTGTTAGGTGTACATGCATCTTCCCCAGACGGACAACAAAAAGAAAATCATCATCACAGAAGATGGAGAGTTTAAAGCCCTGCAGGAGTG[G/A]ATCCTGGAAACAGATGGAGTCAGTCTCATGAGGGTCCTTAGTGAAAAGGATGTGGACCCTGTTCGGACCACCTCCAACGACATTGTGGAGATTTTCACTGTAAGAATTGTGCTGGCCTGTCTAAATGTTGTGATATATTTAAGTCCTCAGATCAAACTTAGAAATTTCTACTCTTGGCCTGCAGGTTCTTGGTATTGAGGCAGTGCGTAAGGCTCTGGAAAGAGAGTTGTACCATGTCATCTCTTTTGATGGTTCTTACGTTAACTACCGCCATCTTGCCTTGCTGTGTGACACAATGACCTGCAGGGGTCACTTGATGGCTATCACTCGTCATGGTATCAATAGGCAGGACACTGGACCACTAATGAAGTGCTCTTTTGAGGAGACGGTAAGGTCGACAGTTGACTATTAAATGTTTTAAAGTGGCAGCCAGGATGGTCTTGTAAATGTCTAAATAATTAAAAATGTAGGTGGACGTGTTGATGGAAGCCTCTTCCCAT
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa42026
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
C > A
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000077495 Nonsense 1937 1965 28 28
Genomic Location (Zv9):
Chromosome 12 (position 23926720)
Other Location(s):
Assembly Chromosome Position
GRCz10 12 22450024
GRCz11 12 22571243
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
TCTCCCACTTCCCCCACTTACTCTCCCACTAGTCCCAAGGGCTCCACTTA[C/A]AGCCCCACTTCTCCTGGCTACAGCCCCACCTCTCCAACCTACAGCCCTGC
Long Flanking Sequence:
CCTTCCTACTCCCCCACCTCTCCCAATTACACACCCACCAGCCCCAACTATTCCCCCACTTCTCCTTCATACTCTCCCACTTCTCCATCCTACTCTCCATCCAGTCCTCGCTACACCCCACAGTCTCCCACCTACACCCCAAGCTCACCTTCCTACAGCCCGAGCTCTCCATCCTATTCCCCTACATCTCCAAAATACACACCCACCTCTCCCTCCTACAGTCCCAGTTCTCCAGAGTATACTCCAACATCCCCCAAATATTCCCCTACTTCTCCAAAGTACTCTCCTACATCGCCCAAATACAGTCCCACCTCTCCAACCTACTCCCCAACTACGCCCAAGTACAGCCCCACTTCCCCAACTTATTCTCCAACCTCTCCTACCTACACCCCAACCAGTCCCAAATACTCCCCTACCTCTCCAACTTACTCTCCAACTTCACCGAAATACTCTCCCACTTCCCCCACTTACTCTCCCACTAGTCCCAAGGGCTCCACTTA[C/A]AGCCCCACTTCTCCTGGCTACAGCCCCACCTCTCCAACCTACAGCCCTGCCATCAGCCCAGATGACAGTGATGAAGAAAATAACTAAAGATTGTTTGCGCCGGGCTTTGTCTGACTTCCCTGATGGGCAGTTTGACCCTAGGATAAAGACTGAAGGAAAGCCATCCAGCTGTGATTCACGGGGAAGGCTGAGCAACCTGGGCTCCCATCCTCTTGGTTTCTCTTTTGGACTTCTTGAACTGTTCTTAATTAGCCTCACTAAACAGGCTTCAGTGGTTGCATATGTTATTTCAGAGGTTTGAACTATAAGCCAAACAAGGCTGCCTTTGTACAGACCATTTGGTCACCATGGTGAGGGAGAACCAATAACTCAAAATTGGTGGAAAGATGCAGGTGGCTAACTGCTTTTTGGAGGAAAAAGATCACTTGTAATCCTCTTTCCATCCATCATTTCTATCTCCCTTACTTTTTCTCTAAAATTCAGTGTTGGATGTAAAGGAA
Associated Phenotype:
Not determined