ZMP
bcor
Ensembl ID:
ZFIN ID:
Description:
BCL-6 corepressor [Source:RefSeq peptide;Acc:NP_991189]
Human Orthologue:
BCOR
Human Description:
BCL6 corepressor [Source:HGNC Symbol;Acc:20893]
Mouse Orthologue:
Bcor
Mouse Description:
BCL6 interacting corepressor Gene [Source:MGI Symbol;Acc:MGI:1918708]
Alleles
There are 8 alleles of this gene:
Allele Name | Consequence | Status | Availability |
---|---|---|---|
sa24091 | Nonsense | Available for shipment | Available now |
sa37445 | Nonsense | Mutation detected in F1 DNA | Not yet available |
sa43784 | Nonsense | Mutation detected in F1 DNA | Not yet available |
sa32384 | Nonsense | Available for shipment | Available now |
sa24092 | Essential Splice Site | Available for shipment | Available now |
sa37446 | Essential Splice Site | Mutation detected in F1 DNA | Not yet available |
sa12397 | Nonsense | Available for shipment | Available now |
Mutation Details
Allele Name:
sa24091
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Mutation:
C > A
Consequence:
Nonsense
Transcripts:
Transcript ID | Consequence | Amino Acid Affected | Amino Acid Total | Exon Affected | Exon Total |
---|---|---|---|---|---|
ENSDART00000047442 | Nonsense | 355 | 1777 | 3 | 13 |
ENSDART00000102106 | Nonsense | 355 | 1796 | 3 | 14 |
Genomic Location (Zv9):
Chromosome 22 (position 11265620)
Other Location(s):
Assembly | Chromosome | Position |
---|---|---|
GRCz10 | 22 | 11125788 |
GRCz11 | 22 | 11155470 |
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
TAGTACTATGGAGTTCCAGAAGCCTCTGTACAGAAGTCCTTCCTCATCCT[C/A]ATCATCTTCACCATCAGTATCTCACCCTGTCTATATTAGCAGTGCATCCC
Long Flanking Sequence:
TTACATGCCCACCTATCCCCCCGCACCTATATCTTCACCTCTTGCCCCTCCGCTCAGGATCCCACCTGCAACCGTAGCCCCTACTACATTGTCACCTATGATGCACCACCAAGACAAAACAGTACAGAGTATTGGTCCAAGGATACACCATGAGCCCTCAGCATTTGGGCAGCAGACCATACATCAGCACACTCAGCCCCACCACCAGTCACCCATTGATAGACAGCACAGCAGCAGTGGAAACAGTTCAAGCAACAGCAAGTCGATCCGGACCTCATCCAGCAAAAACTTAAGTAGCACTAGCAGTATTGTTAGTAGCAGTGCTGGTATTAGCAGCTGCACCAGCTCCATAGTCTCAGTAGACTCATGTCCTTCCCTTGTAATGCAACCACCACGCCCTACACCACGCCCCCCTCAGCCACCTGCAGCACCTCCACCTCCTCTTATGGATAGTACTATGGAGTTCCAGAAGCCTCTGTACAGAAGTCCTTCCTCATCCT[C/A]ATCATCTTCACCATCAGTATCTCACCCTGTCTATATTAGCAGTGCATCCCAAGAGCTTCGCTCTCCAATTCGGTCCTCTGGTCAAAAGCCTAAAGCCAAAGAAGCAACCATGGAGTCTACCAGAGGTATGGGGAATGAAAGAAAGGGAAGCAGCTCACCTGTTAAGACCTCTTCTGATAAATCACCTCAACAGGGGCCTATTACCAAAGATCCAGCAGACAAACCTTTAGACCTGTCTGCCAAAATCATGGAATTTGAAGGGCATACTAATGGGTACCCCTCAAAATTAGAAGCCTTAGCCAAGCTTGGATATTCTCCTGCTGCACGTTATGGACTTCCCCCAAACAGGGAGCTTTTAAAAGAAACTCTTTCTCCATCATCCTCCGCTGTTAGCACTTCTAAAACTCCAGAAAGGCCTGAAATAATTAGCACTTTACCCTCTTCCTGGGTTGTGCCAAGTCCTACCCAGACAATCAGCTCTGAGGCTAGTCAAAACAAAG
Associated Phenotype:
Not determined
Mutation Details
Allele Name:
sa37445
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
T > G
Consequence:
Nonsense
Transcripts:
Transcript ID | Consequence | Amino Acid Affected | Amino Acid Total | Exon Affected | Exon Total |
---|---|---|---|---|---|
ENSDART00000047442 | Nonsense | 758 | 1777 | 3 | 13 |
ENSDART00000102106 | Nonsense | 758 | 1796 | 3 | 14 |
Genomic Location (Zv9):
Chromosome 22 (position 11266830)
Other Location(s):
Assembly | Chromosome | Position |
---|---|---|
GRCz10 | 22 | 11126998 |
GRCz11 | 22 | 11156680 |
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
CTGAGGCCCAGAACCCTGGACAAGACTTGGCACCATGATGAGCCTCCTTA[T/G]AAGCGCCAGAGCATCACTGACATAGACCCAGACTACAAATCTGAGAAGGA
Long Flanking Sequence:
ACCCCAATAAATAACCACAGTGGCAGCCACTCCTCAGCTACAAAACCTTCAAAGACAGCCAAAAAAACTGAAACCCCTGATATTGGCTTTAAGCCCCAGCAATCACATTTAGAGAATGGACATACACCAAGTCACCTCTACATGCAACAGGGTGAAACTTATCTTTCTCCCGGCTTAGCATATTCTAGCAGATATCTCTCATACCCAGTACCTGAGAGCCTTTCCCTTTCCCACTTGCAGTTGTCAGGAAAAGGTCCAGTGTATCCTCATCCAGTTCTGCTGGGCGCTAACAGCCTGTATTCAACACGTTTGCCACCCAAGCCTGGCATTCCTTTTGGAATCCCACCCAATCATGCGGAATATCTTACCTACCATGACTCACAAGAGATGGTCCATCCACTAATGTCTCCCCACCTGCCCCTTGACCACAAAGTAACTGAACGCCTGGATCTGAGGCCCAGAACCCTGGACAAGACTTGGCACCATGATGAGCCTCCTTA[T/G]AAGCGCCAGAGCATCACTGACATAGACCCAGACTACAAATCTGAGAAGGAATCAGAGAGGCAGGAAGTTTTGGGCTCAAAGTCTCTGAGCAAGCCTCACACAGTGAATAAAGATGAGATTGTCTGCATCGACCTGGTCCAAGATGACACAGATGGCAGTCCAGAGCCAGACAAACATAGTACCATCGATGCCAAATGCAAAGAGCCAGCTAAGCCAGTAGGCAGTGGGACAGGGATGGGGAATGAGAGTGGCAGTAATTCTGAGGGGAAAGAGCCAGAGCTCATGCAGATCTTGCGCTCTGGCCAGCCTGCTGTATCTTGGCCAACTGATTCAGAGCAACGGGCAGAGGTATGCAGTCCACCTCGGCCACATAAGCAGTCTGACTCTCCTTTGCACAGCCAGAGTGAGGAAAGCTCCTCTGAGCATAGCCCACTACCTGACATGTTGGAAGAGCAGACACTACGCTGTGCCAGAACCTCTGGTGAAAGGGCTCGAGCTGA
Associated Phenotype:
Not determined
Mutation Details
Allele Name:
sa43784
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
C > T
Consequence:
Nonsense
Transcripts:
Transcript ID | Consequence | Amino Acid Affected | Amino Acid Total | Exon Affected | Exon Total |
---|---|---|---|---|---|
ENSDART00000047442 | Nonsense | 938 | 1777 | 3 | 13 |
ENSDART00000102106 | Nonsense | 938 | 1796 | 3 | 14 |
Genomic Location (Zv9):
Chromosome 22 (position 11267368)
Other Location(s):
Assembly | Chromosome | Position |
---|---|---|
GRCz10 | 22 | 11127536 |
GRCz11 | 22 | 11157218 |
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
GGGCTCGAGCTGATGGAGCTGAATTCAGAGTTGATCGGCACCACACTAAT[C/T]GACAATATGTGGACCTGGGCAAGGATGGACTTGAAGACACAGAGTCCCAT
Long Flanking Sequence:
AATCTGAGAAGGAATCAGAGAGGCAGGAAGTTTTGGGCTCAAAGTCTCTGAGCAAGCCTCACACAGTGAATAAAGATGAGATTGTCTGCATCGACCTGGTCCAAGATGACACAGATGGCAGTCCAGAGCCAGACAAACATAGTACCATCGATGCCAAATGCAAAGAGCCAGCTAAGCCAGTAGGCAGTGGGACAGGGATGGGGAATGAGAGTGGCAGTAATTCTGAGGGGAAAGAGCCAGAGCTCATGCAGATCTTGCGCTCTGGCCAGCCTGCTGTATCTTGGCCAACTGATTCAGAGCAACGGGCAGAGGTATGCAGTCCACCTCGGCCACATAAGCAGTCTGACTCTCCTTTGCACAGCCAGAGTGAGGAAAGCTCCTCTGAGCATAGCCCACTACCTGACATGTTGGAAGAGCAGACACTACGCTGTGCCAGAACCTCTGGTGAAAGGGCTCGAGCTGATGGAGCTGAATTCAGAGTTGATCGGCACCACACTAAT[C/T]GACAATATGTGGACCTGGGCAAGGATGGACTTGAAGACACAGAGTCCCATGAGGATGAGGAGGAAGGACATGGATGTTCTAAAGGAAAGAGATCCAGCTTGGCCAAGAGAATAGCCAACTCCTCAGGCTATGTTGGCGACCGCTTCAAGTGCGTCACCACAGAGTTGTATGCAGACTCCAGCAAACTCAGCCGGGAACAGCGAGCCTTGCAGGTGAGTTGTACAATTTTTGTATGTTAGTCAAGATGTACAGGGAAGTTTTTCCAGTTATGGAGCATGTCACATTGTCACACTATGTTTGAGAATCATCTAAGCATCCTATGGTCAAGTTAAAGTGCAACAATATCAAAACACCTTAATGTCCTGTTCCATGGTCAATACTTTACAGACTTTACAGCAATTGGTGTTATTGCTTTAGTTCAAGGCCATCCTGAGATTTACAGCTTCTTTCTGAGACCTTAGTTCCAGCTTTGAATGAATACTGATATCTGACTTCCACAG
Associated Phenotype:
Not determined
Mutation Details
Allele Name:
sa32384
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Mutation:
T > A
Consequence:
Nonsense
Transcripts:
Transcript ID | Consequence | Amino Acid Affected | Amino Acid Total | Exon Affected | Exon Total |
---|---|---|---|---|---|
ENSDART00000047442 | Nonsense | 981 | 1777 | 3 | 13 |
ENSDART00000102106 | Nonsense | 981 | 1796 | 3 | 14 |
Genomic Location (Zv9):
Chromosome 22 (position 11267499)
Other Location(s):
Assembly | Chromosome | Position |
---|---|---|
GRCz10 | 22 | 11127667 |
GRCz11 | 22 | 11157349 |
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
AAAGGAAAGAGATCCAGCTTGGCCAAGAGAATAGCCAACTCCTCAGGCTA[T/A]GTTGGCGACCGCTTCAAGTGCGTCACCACAGAGTTGTATGCAGACTCCAG
Long Flanking Sequence:
GACAAACATAGTACCATCGATGCCAAATGCAAAGAGCCAGCTAAGCCAGTAGGCAGTGGGACAGGGATGGGGAATGAGAGTGGCAGTAATTCTGAGGGGAAAGAGCCAGAGCTCATGCAGATCTTGCGCTCTGGCCAGCCTGCTGTATCTTGGCCAACTGATTCAGAGCAACGGGCAGAGGTATGCAGTCCACCTCGGCCACATAAGCAGTCTGACTCTCCTTTGCACAGCCAGAGTGAGGAAAGCTCCTCTGAGCATAGCCCACTACCTGACATGTTGGAAGAGCAGACACTACGCTGTGCCAGAACCTCTGGTGAAAGGGCTCGAGCTGATGGAGCTGAATTCAGAGTTGATCGGCACCACACTAATCGACAATATGTGGACCTGGGCAAGGATGGACTTGAAGACACAGAGTCCCATGAGGATGAGGAGGAAGGACATGGATGTTCTAAAGGAAAGAGATCCAGCTTGGCCAAGAGAATAGCCAACTCCTCAGGCTA[T/A]GTTGGCGACCGCTTCAAGTGCGTCACCACAGAGTTGTATGCAGACTCCAGCAAACTCAGCCGGGAACAGCGAGCCTTGCAGGTGAGTTGTACAATTTTTGTATGTTAGTCAAGATGTACAGGGAAGTTTTTCCAGTTATGGAGCATGTCACATTGTCACACTATGTTTGAGAATCATCTAAGCATCCTATGGTCAAGTTAAAGTGCAACAATATCAAAACACCTTAATGTCCTGTTCCATGGTCAATACTTTACAGACTTTACAGCAATTGGTGTTATTGCTTTAGTTCAAGGCCATCCTGAGATTTACAGCTTCTTTCTGAGACCTTAGTTCCAGCTTTGAATGAATACTGATATCTGACTTCCACAGTCGCTTATATTGAACTCCTCGCTAATTACAGATCTCTGTAACCCACTCTCCCTCTCCTGCTCTCTCTTTCTAGCAGTTTTTGGGCCCCTGCTGTGAATCTGTCAAACAGGCTTTAATAGCAGCAGTTCTAC
Associated Phenotype:
Not determined
Mutation Details
Allele Name:
sa24092
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Mutation:
A > T
Consequence:
Essential Splice Site
Transcripts:
Transcript ID | Consequence | Amino Acid Affected | Amino Acid Total | Exon Affected | Exon Total |
---|---|---|---|---|---|
ENSDART00000047442 | Essential Splice Site | 1630 | 1777 | 12 | 13 |
ENSDART00000102106 | Essential Splice Site | 1649 | 1796 | 13 | 14 |
Genomic Location (Zv9):
Chromosome 22 (position 11302984)
Other Location(s):
Assembly | Chromosome | Position |
---|---|---|
GRCz10 | 22 | 11163152 |
GRCz11 | 22 | 11192834 |
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
ATTCACACCTTGTCTCTCATTTCTAATCGATATCTGTCTCTCTGACTCAC[A/T]GAGCCTGCCGACGACTCGTCTGGGTACGATATCCTGGCCAACCCTCCCGG
Long Flanking Sequence:
GTGAGTATGTGTGTGTGTGTGTGCGCGCGGGCCTGTCTCCAGCTGGCCAAAAACAAACCCACTGCTACTGCACATTTTCCCTTGGATTCCTGGAATAACAAAGCCGGGAGAGAAGGGGAGGTGTAGATGGGAGCCGCGCGGCTATTTTTGGGAGCTTTGCACCGCCTCGGAAAAACCCAAAGCGTAACAGAAAGAGGCAGAGGCGCCGGTTTCGCTCCGAGGCGTACGCTGTGCCACTAGCGCGCACGAAACGACTGAAGTGGCTTCACACGCAGCTCTTCCCGCGGTGCTGCTTAATGGAAGAGAGTGCAAATAGGAGAGCGACGTGAGAGTAGCGGCGCAAAAGCATGTCTCGGCGTGAATCCGACAGGGAGAGGGAGGGAGGGCGGAAGGGGATATTATGGGAGCTCAATCCATTCCGCGCTCTCAGAGAGTGTGTGCGCTTCCATAATTCACACCTTGTCTCTCATTTCTAATCGATATCTGTCTCTCTGACTCAC[A/T]GAGCCTGCCGACGACTCGTCTGGGTACGATATCCTGGCCAACCCTCCCGGACCCGGGGAAGACGAGGATGAGCAAAGAGATGTTTTCGAATTCGAGTTCTCAGACCGACCGCTGCTGCCGTGCTACAACATCCAGGTGTCTCTCTCACAGGGGTAAGAGTCTAAAGTTTTTGACCTTTTGTTCTTAACTGCTTTATAAAGGTATTTCGTTTGAGGGGCTAATAGTGATTCTCAGGTGAGAATCGATTCTGAGTGGACTCAATGCTGGCCTGAAGTGTTCCAAATGTGGGAACATGTTTGAGATTCCAATTCCGAGTAGAACACTTGTAATTGGTTTAAAGTCTGCGTGAACTGGAAGTTGCTGAGACTTTTCATATGTTTATGTACTTCCAAATTAAATAAGTATTAAGTAGATGGCAGGGTCCTTCTTTTTGCACATCATTCCCTCAGCAAACTAATGTTAAGGAGGAATAGTTAAGAATATTATGGCTGAAGCTGTCA
Associated Phenotype:
Not determined
Mutation Details
Allele Name:
sa37446
Status:
Mutation detected in F1 DNA
For more information about the meaning of this status and other statuses, please see our FAQs.
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Not yet available
Mutation:
A > C
Consequence:
Essential Splice Site
Transcripts:
Transcript ID | Consequence | Amino Acid Affected | Amino Acid Total | Exon Affected | Exon Total |
---|---|---|---|---|---|
ENSDART00000047442 | Essential Splice Site | 1680 | 1777 | None | 13 |
ENSDART00000102106 | Essential Splice Site | 1699 | 1796 | None | 14 |
Genomic Location (Zv9):
Chromosome 22 (position 11309608)
Other Location(s):
Assembly | Chromosome | Position |
---|---|---|
GRCz10 | 22 | 11169776 |
GRCz11 | 22 | 11199458 |
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
TTGCTCTCATTTTCAGCTAATTAATCTCTCTTTCTCCCGCTCTTGTTCAC[A/C]GACCCAGAAATTGGTTGCTGCTCTCAGATGTCCTCAAGCGACTGAAGATG
Long Flanking Sequence:
TATTTTACAGAGGAGCACCAAAAGCATTTTTTGGCTCCACCCACTTGTAAAAGATTGGTTATTATAAGATTGTAGACTACAAATGTTAGTCCAAAATGTTGGTTTAGGAGTAAGTTTGTTCCAACATTAAGCAACTTCCCATCTTGTATAGTCATCATGTGCATATATATTGTTTCTGTAGCTACAATCAACAACCAATCGTAGTGCTTCATTTCCCTCATTATAGATATTTCTTTAATCTTTGTTTTTATCTAATTGTAGTTTTCATTCCTTGCTTCAAATCTAGTGTGCAATGTCATGATTTATTTTGCAGCTTGTTGGTTTGGTTCATGGCTTATAACTGTTTGACTACACCTAATCCCTGTGGGAAAAATATTTCTGGAACCAGTGCTGCTAAAAAAAACAACAAAAGTAGCGCAAACTAGCCACTGTGACGCTAGCGTGAAAACCTTGCTCTCATTTTCAGCTAATTAATCTCTCTTTCTCCCGCTCTTGTTCAC[A/C]GACCCAGAAATTGGTTGCTGCTCTCAGATGTCCTCAAGCGACTGAAGATGTCTGCCAGAACGTTTCGTGCCACGTTCACGCACATCGAGGTGGTGACGATAGCGGAGGCTGAATTCTACAAGCAGGCGTCTCTGAGTCAGCTGTTCTCCTGTCCGGAGGAGCTCGAGGGATTCATGCCTGACAGCAAGGAACTGTTGGATCTGGTGGAAATCAGCGGCGAGCTGGTGGCGCTGCTGGGGTCATCGATTGAATGCTTGGACGACCGCTGGGAACCCGCTTCCAGACCTCGCTCCTGAACTATTGCACACGCACTCGGACAGACCCGTCGAGACCAGTGGAGAACTAGAAGCAAAGGATGAGGATATTCTGAGGCAGTTCTTGACATTTCAAGGAACTTCTGGATCGGTAGCCAAAGGCGAAATTGGGTCCCAGTGCAGCTGGTATTTCTGTTTGGCTACGAGGAGGTTTCAGAACATCTCGACATCTCTTGTGCTCTGCTC
Associated Phenotype:
Not determined
Mutation Details
Allele Name:
sa12397
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Mutation:
G > T
Consequence:
Nonsense
Transcripts:
Transcript ID | Consequence | Amino Acid Affected | Amino Acid Total | Exon Affected | Exon Total |
---|---|---|---|---|---|
ENSDART00000047442 | Nonsense | 1770 | 1777 | 13 | 13 |
ENSDART00000102106 | Nonsense | 1789 | 1796 | 14 | 14 |
Genomic Location (Zv9):
Chromosome 22 (position 11309878)
Other Location(s):
Assembly | Chromosome | Position |
---|---|---|
GRCz10 | 22 | 11170046 |
GRCz11 | 22 | 11199728 |
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
AGCTGGTGGCGCTGCTGGGGTCATCGATTGAATGCTTGGACGACCGCTGG[G/T]AACCCGCTTCCAGACCTCGCTCCTGAACTATTGCACACGCACTCGGACAG
Long Flanking Sequence:
CTTGCTTCAAATCTAGTGTGCAATGTCATGATTTATTTTGCAGCTTGTTGGTTTGGTTCATGGCTTATAACTGTTTGACTACACCTAATCCCTGTGGGAAAAATATTTCTGGAACCAGTGCTGCTAAAAAAAACAACAAAAGTAGCGCAAACTAGCCACTGTGACGCTAGCGTGAAAACCTTGCTCTCATTTTCAGCTAATTAATCTCTCTTTCTCCCGCTCTTGTTCACAGACCCAGAAATTGGTTGCTGCTCTCAGATGTCCTCAAGCGACTGAAGATGTCTGCCAGAACGTTTCGTGCCACGTTCACGCACATCGAGGTGGTGACGATAGCGGAGGCTGAATTCTACAAGCAGGCGTCTCTGAGTCAGCTGTTCTCCTGTCCGGAGGAGCTCGAGGGATTCATGCCTGACAGCAAGGAACTGTTGGATCTGGTGGAAATCAGCGGCGAGCTGGTGGCGCTGCTGGGGTCATCGATTGAATGCTTGGACGACCGCTGG[G/T]AACCCGCTTCCAGACCTCGCTCCTGAACTATTGCACACGCACTCGGACAGACCCGTCGAGACCAGTGGAGAACTAGAAGCAAAGGATGAGGATATTCTGAGGCAGTTCTTGACATTTCAAGGAACTTCTGGATCGGTAGCCAAAGGCGAAATTGGGTCCCAGTGCAGCTGGTATTTCTGTTTGGCTACGAGGAGGTTTCAGAACATCTCGACATCTCTTGTGCTCTGCTCGCATACTAATTCTCCCGGTGACGATGCAAACCTGTACATAGTGTACATTCTGCAGATACGAAAAGAGAACGGACCTAAGATATTTGGACCACTATCACTGGCTAATTCAGAGATATGAAGCCATGCTCGCGATGATGAACTCTTTCATTTAAAAGAGATGTTTTCTAAATAGGTATATAAGTATATATATATATACAGTCACTGTATTTTATTTGTGTGTATTATATATAGATATAAATATATATGAATATAAATAAATAAATATATAAA
Associated Phenotype:
Not determined