Busch Lab

ZMP

si:dkey-1h6.1

Ensembl ID:
ENSDARG00000018266
ZFIN ID:
ZDB-GENE-041001-127
Description:
Novel protein similar to vertebrate methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methe
Human Orthologue:
MTHFD1
Human Description:
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase
Mouse Orthologue:
Mthfd1
Mouse Description:
methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase,

Alleles

There are 8 alleles of this gene:

Allele Name Consequence Status Availability
sa32295 Nonsense Available for shipment Available now
sa1702 Essential Splice Site Available for shipment Available now
sa32296 Essential Splice Site Available for shipment Available now
sa23722 Nonsense Available for shipment Available now
sa14993 Nonsense Available for shipment Available now

Mutation Details

Allele Name:
sa32295
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Order Allele From ZIRC
Mutation:
T > A
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000008199 None None 935 None 30
ENSDART00000141697 Nonsense 14 900 2 26
Genomic Location (Zv9):
Chromosome 20 (position 27677085)
Other Location(s):
Assembly Chromosome Position
GRCz10 20 27748388
GRCz11 20 27647478
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
GTGTGCAGACATGTTTTCTGTCAGTGCTGCTGTGCTACGGCGAGGTGTTT[T/A]AAGGTACAGATGGGACAGATGCTGTGCGCTTGCAACAGTTGTTTCTGGAA
Long Flanking Sequence:
GCTTAGCAGATCACATGCTGCAGTTTTTTGACGGCGAAGAAGAGGAGGAGACCGACTAAACTCACAGTCCCAGGAATGTTTCTTTTCTCACCCACAAGACCTCTGATTGGCTGCCTGCTCCTCGCTGAACAGCCTACCTGCTCCACTTGCCAGTAGTTGTATTTTAACCTCTCTTGCATTGTGCAGGCTTTTTCCACAAACCATGTGATTGTTTGAGCTTGTCTGAGTTTTGCAGCCTCTACTTTTATCAGCTCACATCCCGACTCGTGCTGGAATAGTCTGGACAGCTGGATGCATTGATAAGACTGCACAGTGAAAGCAGGAGGAGTGACTGTACTGACTTCATACAGTTCATACAATACAGGTGGTGATATCTCTGTGATGTAGAGAAACAGCTTTGTTTGAAGATTTTTTTCCTGTATGTTGTCCAACTGACTTTGACACTGTGTTGTGTGCAGACATGTTTTCTGTCAGTGCTGCTGTGCTACGGCGAGGTGTTT[T/A]AAGGTACAGATGGGACAGATGCTGTGCGCTTGCAACAGTTGTTTCTGGAAACAAGACCTCACAGTGAGTTTGAGTGTGTTTGAGGGCTCTTGTTGATGTAGGACTCAGGAGCTTTTACACATTATCAAGCATTTGATGCTTTTTAAATTTAGTTTATTGTGTTTATTCTAAGCCTTTTAACAATATGCTTGTAATATAATAATGTACTTAATTTAAAATGGAAACACCTTATAATAACTATTCATTTGTAAACCTTGGGAACTTACAATATAAAGTGCTCAGCATAATTGAATACACTCCATTTTGAAAATAAATATTTTTATCCATTTCTCTGTGAATATAGGCAATGTATTTTGGTGCCCTAAACAAAACCAATTTATTAAACATCTATATTTATTAAAATAATATTTATTTTATGCACCAATATTTAGCCACCAAACATATTTAGGAAGTGAAAGATAATACAATTAAGTGCAAGCTAAATTATTATTTTTTTTATC
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa1702
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Order Allele From ZIRC
Mutation:
T > A
Consequence:
Essential Splice Site
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000008199 Essential Splice Site 80 935 4 30
ENSDART00000141697 Essential Splice Site 101 900 5 26
Genomic Location (Zv9):
Chromosome 20 (position 27681819)
Other Location(s):
Assembly Chromosome Position
GRCz10 20 27753122
GRCz11 20 27652212
KASP Assay ID:
554-1648.1 (used for ordering genotyping assays)
KASP Sequence:
AATCAATGCGAACCACATCAGGCTTCCTGAAACAGCCACTGAAAATGAAG[T/A]AAGAGAAACACAATAAATAACAACGTTTATCCATATATCATTGTTTTAAT
Long Flanking Sequence:
ATATGCCGTAATAGTTGACAAAGCCAAAGGAAAATTAATAAATTAATTTATTTAAAATCACTCAGAAATGTTGCTTAACACTATTAAATATTTAAATATTTTCACAAAATAAAATATTTCTAATTAAACCCTTGCAAAACAAATATCTGCACAAAAATTTCTAATTGCATTTTTATATTCATAAACAGCAACATTTTGAAGATTAAGTAGTTTTCTTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTCAAAGGTTGGAGACAGAGAAGACTCAAACCTCTACATCAGTATGAAGCTCAAAGCTGCGGCTGAGGTTCAGTGAGCTGATTTAATAAACTGAAGTCTTGTTTATCATAAACCTCTCACTCACACTGTCTCTCTCCTTGGTTTAGATTGGAATCAATGCGAACCACATCAGGCTTCCTGAAACAGCCACTGAAAATGAAG[T/A]AAGAGAAACACAATAAATAACAACGTTTATCCATATATCATTGTTTTAATCTTAATTAGAAGTCTTTTGTTTTAATCAACGCTGAAAAAATACAGCAACCAATCTATTGCAAAGCCATCATAGAAAAATGATGAAAGATCCAATGCCCTCATCAGTTTGATAGAGTTTATCCTTTAAAATTACAAAACAGTCTGTCTGGGTTAATTGTAACACCTTTATTACATTTCCTAAACCACCTACTGACTAACCTTCAGTTCCGATTTAGTTTTGTAAGGAAGTTGCAATTGATCATTAGTATAATTAATAAATGTCGCACACAGCAGATTAAACTATTTTGTGAGAAATATGATTTAAGGACTGGTCTTCTTTAGGTCCTACGCAACATAGTTGCGGTGAATGAGAATCCTGCATTTCATGGGCTCATTGTGCAGCTGCCGCTCGACTCCATCAACCCAATAAACACTGAGAAAATCACCAATGCTGTCGCTCCAGAGAAAGAT
Associated Phenotype:
Normal
Stage Entity Quality Tag
Larval:Day 5
ZFS:0000037		 whole organism
ZFA:0001094		 quality
PATO:0000001		 normal
PATO:0000461

Mutation Details

Allele Name:
sa32296
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Order Allele From ZIRC
Mutation:
T > C
Consequence:
Essential Splice Site
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000008199 Essential Splice Site 725 935 23 30
ENSDART00000141697 Essential Splice Site 716 900 22 26
Genomic Location (Zv9):
Chromosome 20 (position 27701910)
Other Location(s):
Assembly Chromosome Position
GRCz10 20 27773213
GRCz11 20 27672303
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
TTCATAGATCCAAGCAGGTTTGCCCCTTCCAAAAGAGTACTTACAAGAGG[T/C]AATACACTTTTACTACTGACAACATTGTGATAGATAGATAGATAGATAGA
Long Flanking Sequence:
TTTAATATTTTATAACAGCTATTTTACTTTTGATCAAATGAATGCATACTAGCATATGAGAGTTTTTTTTTCAGAGGCACTGAAAAATATTACTAACTTCAAACTTACATTAAAATATAAAAAATATGGTCAGATTTCTGTAATATCAACAACAAAAATATAATCTGGTTCTATCTGTATTTCAGTCACAGAGTCGGGTCATGGAGCTGATGTTGGCTTGGAGAAGTTTTTCAACATTAAATGCCTCTACTCAGGTCTTCAGCCAGATGTGCTAGTGATGGTGGCAACCATTCGTACATTGAAGATGCAAGGTGGAGCACCTCCTGTGAGTAACACAACACACATTCATAATGGATTGGTTAGCCATTTAAAACTATTAGACATCAACACATCCATTGTGTTCATTAAAATATCTGTTTTGTGCTTCAGACTGCAATTATATTCTTTCTGTTCATAGATCCAAGCAGGTTTGCCCCTTCCAAAAGAGTACTTACAAGAGG[T/C]AATACACTTTTACTACTGACAACATTGTGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGACTATGCATGTTCCTATTACCTGTTTAAACACAAATGTTTTTTTATACACATTTATTCTATATTTGAAGTTTTAATAAACTTGCATAACATATAATAATTGTAATACATAACACATTATACATAAAACCATATGAAATAAATAATGTAGATGCTATTAAAATTGTACATAATGGATATACATTTTTCTATTGGCTTCAGCACACACATATCTGAAA
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa23722
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Order Allele From ZIRC
Order Allele From EZRC
Mutation:
A > T
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000008199 Nonsense 757 935 24 30
ENSDART00000141697 Nonsense 748 900 23 26
Genomic Location (Zv9):
Chromosome 20 (position 27702725)
Other Location(s):
Assembly Chromosome Position
GRCz10 20 27774028
GRCz11 20 27673118
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
None
Flanking Sequence:
AGGTGGAAAAAGCACAAGCATTCGGCTTGCCGGTGATCGTAGCTGTCAAC[A/T]AATTCAGGTAAAGCAGCAAGCAGCCAACAATGAAATATTTTGCTATTTGA
Long Flanking Sequence:
ACAAATGTTTTTTTATACACATTTATTCTATATTTGAAGTTTTAATAAACTTGCATAACATATAATAATTGTAATACATAACACATTATACATAAAACCATATGAAATAAATAATGTAGATGCTATTAAAATTGTACATAATGGATATACATTTTTCTATTGGCTTCAGCACACACATATCTGAAAACTATATGTAGCCTTCTATCTCACAAGGTCACTATTGATATAGGTCAATATGGCATATAATAGAGTTTTGAATGGTGTAGTATTTCAAATATATTTAGAACCTAATTAACTGACAAATGTGGTCAATTGTTTTATGTACACTTATTGACTGTAGGAGACACTGCTGTTTAGATTGATTTAGTGTGTGCATCACTTGAAGTGAAGTATTTTCTCCAATGCAGAACCTCAAGCAGCTGGAAAGAGGCTGTTGTCATATGAGGAGGCAGGTGGAAAAAGCACAAGCATTCGGCTTGCCGGTGATCGTAGCTGTCAAC[A/T]AATTCAGGTAAAGCAGCAAGCAGCCAACAATGAAATATTTTGCTATTTGAGACTTTTCATACATTATCATTAGAATAAAATAGATTATCTTTAATGTCATTGCATGGAGTATACAACCAAATTTGCTCGACAAAAATTTTGGTTTGATCAAGTTAAAGTTTAGTTAGTCAATATTAAAACATATTTCAAGTGATTAAAGATTCATTAAACAATCACGTAAAAAATGTATATGTGGTCAATGTTGTAAATGTAAAACAAAGTTACTTTTTGGAACATCTCTGCAAAAAGGATGCATGGAAAGTGAAGGAAAATATTGTATTAATCTTATAGTAGTGTAAAATCAGAAATAGGGTAAATATTTCTTTAAATTTATATTTAGTCATTAAGCAGACACTTTAGTTCAAAGTGACTTACAATTGAGGAGGCATTCAGTGATTCAACAAGAAGAGGCAATGCACACAAGAAGTTTTAATTAAACAAATGAACTGTTAATGCTAGGA
Associated Phenotype:
Not determined

Mutation Details

Allele Name:
sa14993
Status:
Available for shipment
For more information about the meaning of this status and other statuses, please see our FAQs.
Availability:
Order Allele From ZIRC
Order Allele From EZRC
Mutation:
G > A
Consequence:
Nonsense
Transcripts:
Transcript ID Consequence Amino Acid Affected Amino Acid Total Exon Affected Exon Total
ENSDART00000008199 Nonsense 787 935 25 30
ENSDART00000141697 Nonsense 778 900 24 26
Genomic Location (Zv9):
Chromosome 20 (position 27704789)
Other Location(s):
Assembly Chromosome Position
GRCz10 20 27776092
GRCz11 20 27675182
KASP Assay ID:
None (used for ordering genotyping assays)
KASP Sequence:
GGCCAGGCGAGGCAGGCTGGTGCYTTTGATGCAGTGCAGTGCAGCAGCTG[G/A]TCTGAAGGGGGCGCTGGAGCTCTGGCTCTGGCCGAGACGGTTCAGAGAGC
Long Flanking Sequence:
TAGAATATTAAATAAGGTTTTTAACTGAAACCCTGATCCTTTGAGATGTAAATGCAAGTCAACAGCTTCCAACACTAAAAAAAACATCTACAGGCAAAACAAAATTAATACCCGTGGGTCATGACTTATGGAGCTTATGGAACACAACACTTGGTCTGTTCAAGAAGCTGAACATAATTATTTAATGCAATAGTATTGCATTCATATGAATCCATAAAATCAGAAAAACAACCAGTCCTAATATCTTTCAGTTTTATTCAGTGTCACTGATATATATCTAGTTATGATCTAGTAGATGATTTTTTTTGTCCTTTGTACACTAATCGTATGCTCATGAGCCAAGTTAAACCACACACAACACTCCCACTCTGAGTGAATGTGTGTATGAATATGGACTCTGATTGTGTTTGGCGTAGCTCTGATACTGACGCTGAGCTGGAGCTGGTATGTGGCCAGGCGAGGCAGGCTGGTGCCTTTGATGCAGTGCAGTGCAGCAGCTG[G/A]TCTGAAGGGGGCGCTGGAGCTCTGGCTCTGGCCGAGACGGTTCAGAGAGCAGCAAAACTACCTGCCTGCTTCAAGTTCCTCTATGACTTGCAGGTAAAAAATGGCAAAGTGGGTAATATTTGCACTGATAACCTAAAGGATGCTAAGGCAAGAGATTTATTTTTTTTTTTTTTTTTGGCAAAGAGTAATATCATGCCATATTTTCCCAGTTAATTGATTATCAATGTCAAATATCAAATATTTAAATGCTGTGTTATTTAATTACACATTACACAACTTGTAAATCTAAGCATAAGTTAATTTAATATTTTTATTAGAATTGATTGTTTTTGCATATTAGAGTCGGAGGTGTTAAGGTGAACTTGAGAATCTCCTTTTATCACTTCATAATTTAGAAAATACTGCTAATGGCCTGCAGATACAATAACAACATATGAACATATTTCTGGGTAAAAACCTTCCAGATTTGTGATAAAACACAAAATCGTTTGGGGTATGTA
Associated Phenotype:
Not determined